Hartaetosiga balthica (Wylezich and Karpov) Carr, Richter and Nitsche
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Subculture at peak density (approximately every 10-14 d) to a fresh T-25 flask of fresh medium in the following manner:
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Thomas A Nerad, personal communication