Actinophrys sol Ehrenberg
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Frozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
This strain must be fed with live Chlorogonium elongatum (i.e., ATCC® 50936™ or similar, not provided). The Chlorogonium should be maintained separately and fed to Actinophrys at regular intervals.
This culture is monoxenic, free from bacterial flora.
Periodically add prey organisms as follows:
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If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Kinoshita E, et al. The ultrastructure of contractile tubules in the heliozoon Actinophrys sol and their possible involvement in rapid axopodial contraction. J. Eukaryot. Microbiol. 48: 519-526, 2001. PubMed: 11596916
Sakaguchi M, Suzaki T. Monoxenic culture of the heliozoon Actinophrys sol. Eur. J. Protistol. 35: 411-415, 1999.
diluted to 10% with sterile distilled water