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2. Inoculate a T-25 tissue culture flask containing 10.0 ml of bacterized ATCC medium 802 with 0.1 ml from a predominantly encysted Hyperamoeba culture.
3. Incubate at 25°C with cap screwed on tightly.
4. For routine maintenance subculture every 10-14 d.
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Fiore-Donno AM, et al. Invalidation of Hyperamoeba by transferring its species to other genera of Myxogastria. J. Eukaryot. Microbiol. 57: 189-196, 2010. PubMed: 20113379
Zaman V, et al. Hyperamoeba isolated from human feces: Description and phylogenetic affinity. Eur. J. Protistol. 35: 197-207, 1999.