Entamoeba moshkovskii Tshalaia
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Notes: In general, better growth can often be achieved by using media pre-inoculated with bacteria. Addition of penicillin G at 75 U/mL and streptomycin at 75 µg/mL to ATCC Medium 1171 may be necessary if the bacterial density in the culture is too high. Inoculate fresh tubes of media with bacteria at least one day prior to subcultivation and prior to addition of antibiotics, if used. Also, nutrients in ATCC medium 1171 may be diluted through use of an osmotically-balanced saline buffer such as Dulbecco's PBS (ATCC 30-2200) with the medium. Mixtures in the range of 1:1 to 3:1 (medium to buffer) usually work well to control or limit bacterial growth in cultures where other methods are not sufficient.
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