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Oxytricha trifallax

50532

Product category
Protists
Strain designation
SLC 89
Type strain
No
Product format
Frozen
Storage conditions
-80°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
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Price: $550.00 EA
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Raoultella planticola (Bagley et al.) Drancourt et al.

700831

Price: $376.00 ea

Klebsiella aerogenes Tindall et al.

13048

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Detailed product information

Handling information

Medium
Instruction for complete medium
ATCC Medium 802 inoculated with Klebsiella pneumoniae subsp. pneumoniae (ATCC® 700831™) or Enterobacter aerogenes (ATCC® 13048™). Culture also contains unidentified microalgae as food source.
Temperature
20-25°C
Culture system
Xenic
Incubation
With Enterobacter aerogenes ATCC 13048 and unidentified microalgae as food sources
Handling procedure
Handling of Live Culture
This strain is routinely shipped as a growing culture in a glass 16 x 125 mm screw-capped test tube.  The volume of the cell suspension is approximately 5 mL.  When the culture arrives remove it promptly from the shipping container.  Do not store the culture at refrigeration temperatures before handling.  To assure viability, immediately loosen the test tube cap and incubate upright at 25°C for at least one hour before observing the culture.  There should be numerous active trophozoites in suspension.  If the numbers are low or cysts are present, the culture may have been exposed to temperature extremes in transit.  Regardless of the state of the culture, aseptically transfer a 0.5-1.0 mL aliquot to a 16 x 125 mm screw-capped test tube containing 5 mL of bacterized ATCC Medium 802. Incubate the parent and daughter cultures upright with the caps on loosely at 25°C.
Culture maintenance
Subculture every week to a fresh tube of bacterized medium in the following manner:
  1. Transfer 0.5-1.0 mL from a growing culture to 5.0 mL of bacterized ATCC medium 802. 
  2. Add 1.0 mL of bacterized ATCC medium 802 twice weekly. When the tube is filled to within one inch of the top, aspirate from the bottom of the tube and reduce the volume to 5.0 mL. 
  3. Incubate upright at 25°C with the cap on loosely.
Reagents for cryopreservation
Cryoprotective Solution
DMSO, 2.0 mL 
Fresh growth medium w/o bacteria, 8.0 mL
Cryopreservation

Harvest and Preservation
  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat. 
  2. Harvest cells from a culture that is at or near peak density by centrifugation at 125 x g for 5 min. 
  3. Adjust the concentration of cells at least 1 x 105/mL in fresh medium. 
  4. Mix the cell preparation and the cryoprotective solution in equal portions. 
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. 
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator. 
  8. To establish a culture from the frozen state add 1.0 mL bacterized ATCC medium 802 to the frozen ampule and place it in a 35°C water bath.  Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial. 
  9. Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate onto the surface of an ATCC medium 919 (non-nutrient agar) plate containing an overlay of 15.0 mL of bacterized ATCC medium 802. 
  10. Incubate at 25°C with the cap on loosely. 
  11. Once the culture is established, transfer 0.5 mL to 5.0 mL of bacterized ATCC medium 802. 
  12. Follow the protocol for maintenance of culture.

History

Deposited as
Oxytricha trifallax
Depositors
GA Herrick

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

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