Stephanoeca diplocostata Ellis
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Media: ATCC® Medium 1525 and one of the alternate media listed below, combined in equal parts and inoculated with Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831™) or Enterobacter aerogenes (ATCC 13048™)
Alternate media: ATCC® Medium 1405 HESNW medium, ATCC® Medium 1361 Marine Flagellate medium (both media augmented with two sterile rice grains added to culture flask)
Note about growth media: ATCC Media 1405 and 1361 will each support moderate growth when used on their own, but will support more rapid and dense growth when combined in an equal-parts mixture with previously-bacterized ATCC Medium 1525.
Storage and Culture Initiation
Frozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
DMSO, 2.0 ml
Fresh growth medium w/o bacteria, 8.0 ml
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Leadbeater BS. Developmental studies on the loricate choanoflagellate Stephanoeca diplocostata Ellis. V. The cytoskeleton and the effects of microtubule poisons. Protoplasma 136: 1-15, 1987.
Leadbeater BS. Developmental studies on the loricate choanoflagellate Stephanoeca diplocostata Ellis VI. Effects of silica replenishment on silica impovershed cells. Protoplasma 153: 71-84, 1989.
Leadbeater BS. Developmental studies on the loricate choanoflagellate Stephanoeca diplocostata Ellis I. Ultrastructure of the non-dividing cell and costal strip production. Protoplasma 98: 241-262, 1979.
Leadbeater BS. Developmental studies on the loricate choanoflagellate Stephanoeca diplocostat Ellis IV. Effects of silica deprivation on growth and lorica production. Protoplasma 127: 171-179, 1985.
Leadbeater BS. Developmental studies on the loricate choanoflagellate Stephanoeca diplocostata Ellis. VII. Dynamics of costal strip accumulation and lorica assembly. Eur. J. Protistol. 30: 111-124, 1994.