Proterospongia choanojuncta Leadbeater
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1. Vigorously agitate the flask and aseptically transfer 0.5 ml from a growing culture to a T-25 tissue culture flask containing 10.0 ml of ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC® 700831)
2. Incubate flask at 25°C with the cap on tightly.
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Leadbeater BS. Life-history and ultrastructure of a new marine species of Proterospongia (Choanoflagellida). J. Mar. Biol. Assoc. U.K. 63: 135-160, 1983.