Hartaetosiga gracilis (Kent) Carr, Richter and Nitsche
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Subculture at peak density (approximately every 10-14d) to a fresh T-25 flask of fresh medium in the following manner:
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Leadbeater BS, Morton C. A microscopical study of a marine species of Codosiga James-Clark (Choanoflagellata) with special reference to the ingestion of bacteria. Biol. J. Linn. Soc. 6: 337-347, 1974.
Carr M, et al. Molecular phylogeny of choanoflagellates, the sister group to Metazoa. Proc. Natl. Acad. Sci. USA. 105: 16641-16646, 2008. PubMed: 18922774