Acanthamoeba pearcei Nerad et al.
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Alternate media: ATCC medium 711 optionally inoculated with bacteria as above. (Note that ATCC medium 711 is a richer formula than ATCC medium 997 and will produce denser and faster bacterial growth. Excess bacterial growth can inhibit growth of amoebae.)
Establishing Cultures from Dried State
This strain comes dried on shredded filter paper. Dried samples can remain at room temperature for up to one week. If the culture will not be rehydrated within that period, store at 5°C until processed.
Note: a monoxenic amoeba culture can be established in this manner using any suitable bacterial food source.
DMSO, 1.5 ml
Dryl's solution (or similar), 8.5 ml
Follow the protocol for maintenance of culture.
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Nerad TA, et al. Acanthamoeba pearcei n. sp. (Protozoa: Amoebida) from sewage contaminated sediments. J. Eukaryot. Microbiol. 42: 702-705, 1995. PubMed: 8520585
Stothard DR, et al. The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J. Eukaryot. Microbiol. 45: 45-54, 1998. PubMed: 9495032
Gast RJ. Development of an Acanthamoeba-specific reverse dot-blot and the discovery of a new ribotype. J. Eukaryot. Microbiol. 48: 609-615, 2001. PubMed: 11831768
Marciano-Cabral F, Cabral G. Acanthamoeba spp. as agents of disease in humans. Clin. Microbiol. Rev. 16: 273-307, 2003. PubMed: 12692099