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Spongomonas minima Dangeard

50404

Product category
Protists
Classification
Amoebozoa, Spongomonadida, Spongomonadidae
Strain designation
AZ-3
Type strain
No
Isolation source
Soil from aviary at base of tree; Desert Museum
Geographical isolation
United States; Arizona; Tucson
Product format
Dried
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

Characteristics

Comments
This strain is tentatively placed in this genus because the cells superficially resemble members of Spongomonas based upon features discernable at the light microscopical level.
The strain will probably be assigned to a new genus.

Handling information

Medium
Instruction for complete medium
ATCC Medium 802 inoculated with Enterobacter aerogenes (ATCC 13048)
Temperature
25°C
Handling notes

Additional information on this culture is available on the ATCC web site at www.atcc.org.

While every effort is made to insure authenticity and  reliability of strains on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of cultures.

ATCC recommends that individuals contemplating commercial use of any culture first contact the originating investigator to negotiate an agreement.  Third party distribution of this culture is discouraged, since this practice has resulted in the unintentional spreading of contaminated cultures.

Culture maintenance

1.   Agitate a culture at or near peak density by inverting several times.  Encysted cells must be detached from the flask surface with a sterile cell scraper or cotton swab.

2.   Aseptically transfer a 0.3 ml aliquot to a T-25 tissue culture flask containing 10 ml of fresh bacterized medium.

3.   Screw cap on tightly and incubate at 25°C. 

4.   Subculture every 10-15d.

Cryopreservation
1.   Harvest cysts from several cultures in stationary phase of growth.  Detach cysts using a sterile cell scraper or cotton swab.

2.   Aseptically transfer the cyst suspension to 15 ml plastic centrifuge tubes.

3.   Centrifuge at ~800 x g for 5 min.

4.   While cysts are centrifuging, prepare a 20% solution of DMSO in bacterized ATCC Medium 802.  Cool on ice.

5.   Remove the supernatant and pool the cell pellets to one-half the final volume desired with fresh growth medium.

6.   Combine the cell suspension with an equal volume of 20% DMSO cryoprotectant solution (prepared in step 4) to yield a final concentration of 10% DMSO.

7.   Dispense in 0.5 ml aliquots to 1.0-2.0 ml Nunc vials (special plastic vials for cryopreservation).

8.   Place the vials in a controlled rate freezing unit.  From room temperature cool at -1°C/min to -40°C.  At -40°C, plunge ampules into liquid nitrogen.  Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.).  

9.   Store ampules in a liquid nitrogen refrigerator until needed.

10.          To establish a culture from the frozen state, place a frozen ampule in a 35°C water bath just enough to cover the frozen material.  Allow the ampule to thaw completely (2-3 min).

11.          Immediately after thawing, aseptically remove the contents and transfer to a T-25 tissue culture flask containing 10 ml of fresh  ATCC medium 802 inoculated with Enterobacter aerogenes (ATCC 13048).

12.Screw the cap on tightly and incubate at 25°C.  Subculture every 10-15d.      

History

Deposited as
Spongomonas minima Dangeard
Depositors
TK Sawyer
Chain of custody
ATCC <-- TK Sawyer <-- T.A. Nerad
Type of isolate
Environmental
Year of origin
1991

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

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Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

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