Acanthamoeba stevensoni Sawyer et al.
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Establishing Cultures from Dried State
This strain comes dried on shredded filter paper. Dried samples can remain at room temperature for up to one week. If the cultures will not be rehydrated within that period, store at 5°C until processed.
Note: a monoxenic amoeba culture can be established in this manner using any suitable bacterial food source.
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Sawyer TK, et al. Acanthamoeba stevensoni n. sp. (protozoa: amoebida) from sewage-contaminated shellfish beds in Raritan Bay, New York. J. Eukaryot. Microbiol. 40: 742-746, 1993.
Stothard DR, et al. The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J. Eukaryot. Microbiol. 45: 45-54, 1998. PubMed: 9495032
Kong HH, et al. Mitochondrial DNA restriction fragment length polymorphism (RFLP) and 18S small-subunit ribosomal DNA PCR-RFLP analyses of Acanthamoeba isolated from contact lens storage cases of residents in southwestern Korea. J. Clin. Microbiol. 40: 1199-1206, 2002. PubMed: 11923331