Ancyromonas micra Cavalier-Smith
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Storage and Culture Initiation
Frozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
DMSO, 2.0 ml
Fresh growth medium w/o bacteria, 8.0 ml
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Atkins MS, et al. A survey of flagellate divesity at four deep-sea hydrothermal vents in the Eastern Pacific Ocean using structural and molecular approaches. J. Eukaryot. Microbiol. 47: 400-411, 2000. PubMed: 11140455
Cavalier-Smith T, Chao EE-Y. Phylogeny of choanozoa, apusozoa, and other protozoa and early eukaryote megaevolution. J. Mol. Evol. 56: 540-563, 2003. PubMed: 12698292
Cavalier-Smith T, et al. Planomonadida ord. nov. (Apusozoa): ultrastructural affinity with Micronuclearia podoventralis and deep divergences within Planomonas gen. nov. Protist 159: 535-562, 2008. PubMed: 18723395
Heiss, AA, et al. Clarifying the taxonomic identity of a phylogenetically important group of eukaryotes: Planomonas is a junior synonym of Ancyromonas. J. Eukaryot. Microbiol. 57: 285-293, 2010. PubMed: 20384907