Entamoeba moshkovskii Tshalaia
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Notes: In general, better growth can often be achieved by using media pre-inoculated with bacteria. Addition of penicillin G at 75 U/mL and streptomycin at 75 µg/mL to ATCC Medium 1171 may be necessary if the bacterial density in the culture is too high. Inoculate fresh tubes of media with bacteria at least one day prior to subcultivation and prior to addition of antibiotics, if used. Also, nutrients in ATCC medium 1171 may be diluted through use of an osmotically-balanced saline buffer such as Dulbecco's PBS (ATCC 30-2200) with the medium. Mixtures in the range of 1:1 to 3:1 (medium to buffer) usually work well to control or limit bacterial growth in cultures where other methods are not sufficient.
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Clark CG, Diamond LS. The Laredo strain and other 'Entamoeba histolytica-like' amoebae are Entamoeba moshkovskii. Mol. Biochem. Parasitol. 46: 11-18, 1991. PubMed: 1677159
Clark CG, Diamond LS. Intraspecific variation and phylogenetic relationships in the genus Entamoeba as revealed by riboprinting. J. Eukaryot. Microbiol. 44: 142-154, 1997. PubMed: 9109261