Diaphanoeca grandis Ellis
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Handling of Test Tube Culture on Arrival
This strain is routinely shipped as a growing culture in a glass 16 x 125 mm screw-capped test tube. The volume of the cell suspension is approximately 5 ml. When the culture arrives remove it promptly from the shipping container. Do not store the culture at room temperature before handling. To assure viability, immediately incubate upright at 4-18°C (~10°C is optimal) for at least one hour before observing the culture. There should be numerous trophozoites in the culture: Most are likely to be attached to the glass surface, but others may be actively swimming. If the numbers are low the culture may have been exposed to temperature extremes in transit. Regardless of the state of the culture, vigorously agitate the tube (or rub the inside surface of the tube with a sterile swab) and aseptically transfer a 0.5 ml aliquot to a T-25 tissue culture flask containing 10 ml of ice-cold ATCC medium 1405 and two sterile rice grains. Incubate the parent and daughter cultures at 4-18°C with caps screwed on tightly (keep tube upright during incubation).
DMSO, 2.0 ml
Fresh growth medium w/o bacteria, 8.0 ml
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
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If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Wainright PO, et al. Monophyletic origins of the metazoa: an evolutionary link with fungi. Science 260: 340-342, 1993. PubMed: 8469985
Carr M, et al. Molecular phylogeny of choanoflagellates, the sister group to Metazoa. Proc. Natl. Acad. Sci. USA. 105: 16641-16646, 2008. PubMed: 18922774