ATCC ATCC Logo 0

Prototheca moriformis Kruger

50081

Product category
Protists
Classification
KINGDOM: Plantae
Strain designation
RSP-1216
Type strain
No
Isolation source
Cheese factory wastewater
Geographical isolation
Costa Rica
Product format
Frozen
Storage conditions
-80°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Buy Now
Price: $550.00 EA
Discounts are available for our fellow nonprofit organizations.

Generally ships within 1-3 business days

Documentation

Haven't found what you need?

Customize solution

We tailor our materials for your specific application.

Get application-specific materials

Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

Characteristics

Comments
Neotype.

Handling information

Medium
Instruction for complete medium
ATCC Medium 28
Temperature
20-25°C
Culture system
Axenic
Handling procedure

Storage and Culture Initiation

Frozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen.  If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week.  Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C).  Storage of frozen material at this temperature will result in the death of the culture.

  1. To thaw a frozen ampule, place it in a 35°C water bath until thawed (2-3 min).  Immerse the ampule enough to cover only the frozen material.  Do not agitate the ampule.
  2. Immediately after thawing, aseptically transfer the entire contents to a single 16 x 125 mm screw-capped test tube containing a slant of ATCC Medium 28 or distribute over the surface of an agar plate of ATCC medium 28.  
  3. If a test tube culture has been established, incubate upright at 20-25°C with the cap screwed on loosely.  If a plate culture has been established, wrap the plate with parafilm and incubate inverted at 20-25°C.
Culture maintenance
  1. Transfer cells with an inoculating loop to a tube or plate of fresh agar medium from a growing culture at or near peak density.
  2. Incubate as described in step 3 under the section for establishing a culture.
Cryopreservation
  1. Harvest cells from a culture which is at or near peak density by adding 3.0-5.0 ml fresh ATCC medium 28 broth to the slant or plate and washing cells into suspension.  It may be helpful to rub the surface of the agar with a spread bar or inoculating loop to detach adhering cells.
  2. Adjust the concentration of cells to 2 x 107/ml with fresh broth medium, then dilute to half this concentration by adding an equal amount of a 20% (v/v) sterile solution of either DMSO or glycerol in fresh ATCC medium 28 broth.
  3. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).  The time from mixing of the cell preparation and the cryoprotective solution to the start of the cooling cycle should be no less than 15 min and no greater than 30 min.
  4. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)   
  5. The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials can be stored between -80 and -70°C for no longer than one week.
  6. To establish a culture from the frozen state place an ampule in a water bath set at 35°C until thawed (2-3 min). Immerse the ampule enough to cover only the frozen material.  Do not agitate the ampule.
  7. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and add to a fresh slant of ATCC medium 28 or the surface of an agar plate of ATCC medium 28.
  8. Maintain as described above.

History

Deposited as
Prototheca moriformis Kruger
Depositors
RS Pore
Type of isolate
Wastewater
Year of origin
1983

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Pore RS. Prototheca taxonomy. Mycopathologia 90: 129-139, 1985.

Ueno R, et al. Optimization of heterotrophic culture conditions for n-alkane utilization and phylogenetic position based on the 18S rDNA sequence of a thermotolerant Prototheca zopfii strain. J. Biosci. Bioeng. 94: 160-165, 2002.

Frequently Asked Questions

Need assistance with this product? Contact our Technical Support team.

Telephone

Telephone

US and Puerto Rico
800-638-6597

Outside the US
+1-703-365-2700
hours

Hours of Operation

Monday-Friday
9:30am - 5:30pm
US Eastern Time