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Ureaplasma diversum Howard and Gourlay

49782

Product category
Bacteria
Strain designation
2312
Type strain
No
Isolation source
Female bovine urogenital tract
Geographical isolation
Canada; Ontario
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Biosafety Icon BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Preceptrol
No

Characteristics

Serotype
A
Comments
Experimental infection causing infertility
Identification on embryos with protein A gold

Handling information

Medium
Temperature
37°C
Atmosphere
Aerobic
Handling procedure
  1. Follow instructions as suggested for the culturing of Mollicutes:

                     PROCEDURES FOR PROPAGATING MOLLICUTES:

    1. Open the vial according to the enclosed instructions.
    2. Using a Pasteur or 1.0 ml pipette, withdraw approximately 0.5 to 1.0 ml from a tube containing 5.0 ml. Rehydrate the entire pellet.
    3. Aseptically transfer this aliquot back into the tube.  Mix well.
    4. Make serial dilutions by transferring 0.5 ml from the original tube to a tube containing 4.5 ml.  Repeat process by transferring 0.5 ml from the second to a third tube, etc.  Dilutions are important, not only for titration purposes, but also to keep culture in varying stages of growth.  Many strains will die
      out rapidly once acid or alkaline conditions are reached. It is recommended to prepare several dilutions from the initial tube as the cryoprotectant used in the freeze-drying process often inhibits growth. 
    5. Incubate all tubes aerobically at 37°C. The time necessary for growth will vary from strain to strain. 
    6. Depending on the medium used, growth will be indicated by increased turbidity, a color change, or both.
Handling notes
Ureaplasma diversum has a slower rate of growth than most Ureaplasma species.  The indicator in the first tube will change color to a darker red within 3 to 4 days.  It is especially important to make serial dilutions of this strain, for when alkaline conditions are reached (as indicated by the color change), the culture will rapidly die out unless refrigerated immediately (+4°C) or stored frozen at ‑60°C.  Refrigerated broth cultures may remain viable for periods up to 30 days.  No visible turbidity will be seen.  The color change is the only indication of growth.  Therefore, transfer, freeze, or lyophilize the culture as soon as possible. 

Store vials at freezer temperatures until ready to use.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

Deposited as
Ureaplasma diversum Howard and Gourlay
Depositors
HL Ruhnke
Type of isolate
Animal

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Kreplin CM, et al. The effect of intrauterine inoculation with Ureaplasma diversum on bovine fertility. Can. J. Vet. Res. 51: 440-443, 1987. PubMed: 3453263

Britton AP, et al. Protein A gold identification of ureaplasmas on the bovine zona pellucida. Can. J. Vet. Res. 53: 172-175, 1989. PubMed: 2469532

Doig PA, et al. Experimental bovine genital ureaplasmosis. I. Granular vulvitis following vulvar inoculation. Can. J. Comp. Med. 44: 252-258, 1980. PubMed: 7427772

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