Acremonium chrysogenum (Thirumalachar et Sukapure) Gams
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; overnight rehydration is recommended.
5. Mix the suspension well. Use several drops to inoculate recommended solid or liquid medium.
6. Incubate at recommended temperature.
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Hollander IJ, et al. A pure enzyme catalyzing penicillin biosynthesis. Science 224: 610-612, 1984. PubMed: 6546810
Shen YQ, et al. Levels of isopenicillin N synthase and deacetoxycephalosporin C synthase in Cephalosporium acremonium producing high and low levels of cephalosporin C. Bio-Technology 4: 61-64, 1986.
Zhang J, et al. Carbon source regulation of ACV synthetase in Cephalosporium acremonium C-10. Curr. Microbiol. 18: 361-367, 1989.
Malmberg LH, Hu WS. Identification of rate-limiting steps in cephalosporin C biosynthesis in Cephalosporium acremonium: a theoretical analysis. Appl. Microbiol. Biotechnol. 38: 122-128, 1992. PubMed: 1369008
Zhang J, Demain AL. Regulation of ACV synthetase activity in the beta-lactam biosynthetic pathway of carbon sources and their metabolites. Arch. Microbiol. 158: 364-369, 1992.