Trichoderma harzianum Rifai
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2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; overnight rehydration is recommended.
5. Mix the suspension well. Use several drops to inoculate recommeneded solid or liquid medium.
6. Incubate at recommeneded temperature.
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Limon MC, et al. Increased antifungal activity of Trichoderma harzianum transformants that overexpress a 33-kDa chitinase. Phytopathology 89: 254-261, 1999.