Giardia intestinalis (Lambl) Alexeieff
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Media: ATCC Medium 2695† (Quality controlled freeze-dried lots of this medium are commercially available as ATCC PRA-2695)
†previously ATCC Medium 1404
Alternative Media: ATCC Medium 2155 (Quality controlled freeze-dried lots of this medium are commercially available as ATCC PRA-2155)
Storage and Culture Initiation
Frozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
Immediately after thawing, aseptically transfer contents to a screw-capped test tube containing 13 mL ATCC Medium 2695. Incubate the tube on a 15° horizontal slant at 35°C.
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
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If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Nash TE, et al. Restriction-endonuclease analysis of DNA from 15 Giardia isolates obtained from humans and animals. J. Infect. Dis. 152: 64-73, 1985. PubMed: 2409186
Kabnick KS, Peattie DA. In situ analyses reveal that the two nuclei of Giardia lamblia are equivalent. J. Cell Sci. 95: 353-360, 1990. PubMed: 2384520
Weinbach EC, et al. Respiratory metabolism of Giardia lamblia. J. Parasitol. 66: 347-350, 1980. PubMed: 6248623
Isaac-Renton JL, et al. Characterization of Giardia duodenalis isolates from a waterborne outbreak. J. Infect. Dis. 167: 431-440, 1993. PubMed: 8421176
Gillin FD, Reiner DS. Attachment of the flagellate Giardia lamblia: Role of reducing agents, serum, temperature, and ionic composition. Mol. Cell. Biol. 2: 369-377, 1982. PubMed: 7110136