Prototheca zopfii Kruger

30253 ^™

Product category: Protists
Classification: KINGDOM: Plantae
Strain designation: 48-Y
Type strain: No
Isolation source: Colgate Creek sediment from Baltimore Harbor, Chesapeake Bay
Geographical isolation: United States; Maryland; Baltimore
Product format: Frozen

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Documentation

Product sheet

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed Product Information Permits & Restrictions References

General

Specific applications: degrades petroleum crude oil

Characteristics

Comments: Degrades petroleum.
Petroleum-degradation
Growth on acetate or n-alkanes

Handling information

Medium: ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth
Temperature: 25°C
Culture system: Axenic
Handling notes: Additional information on this culture is available on the ATCC web site at www.atcc.org.
While every effort is made to insure authenticity and reliability of strains on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of cultures.
ATCC recommends that individuals contemplating commercial use of any culture first contact the originating investigator to negotiate an agreement. Third party distribution of this culture is discouraged, since this practice has resulted in the unintentional spreading of contaminated cultures.
Culture maintenance: 1. Aseptically transfer a loopful of material to a fresh plate of ATCC medium 28 and spread evenly over the surface with a spread bar.
2. Subculture every 4-6 weeks when incubated at 25C, or every 6-12 months when incubated at 18C.
Cryopreservation: 1. Harvest cells from a culture that is at or near peak density. Add 2-3 ml fresh ATCC medium 28 broth to each plate and wash cells into suspension.
2. Collect cells by centrifugation at 800 x g for 5 min. Adjust the concentration of cells to 2 x 10⁶- 2 x 10⁷/ml in fresh medium.
3. While cells are centrifuging prepare a 10% (v/v) solution of sterile methanol in fresh broth medium.
4. Mix the cell preparation and the 10% methanol solution in equal portions. Thus, the final concentration will be 10⁶ - 10⁷ cells/ml and 5% (v/v) Methanol. The time from mixing of the cell preparation and methanol stock solution to the beginning of the freezing process should be no less than 5 min and no greater than 15 min.
5. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
6.   Place the vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If the freezing unit can compensate for the heat of fusion, maintain rate at        -1°C/min through the heat of fusion. At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately
      -1°C/min.)
7. The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials can be stored between -80 and -70°C for no longer than one week.
8.   To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.
9.   Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and transfer to a test tube containing 5 ml of ATCC medium 28 broth or to the surface of an ATCC medium 28 agar plate.
10.          Incubate a test tube culture upright at 25°C with the cap screwed on loosely (loosened one-half turn); incubate a plate upright at 25°C. Subculture every 4-6 weeks when incubated at 25C, or every 6-12 months when incubated at 18C.

History

Deposited as: Prototheca zopfii Kruger
Depositors: JD Walker
Type of isolate: Environmental
Year of origin: 1973

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Pore RS. Prototheca taxonomy. Mycopathologia 90: 129-139, 1985.

Koenig DW, Waed HB. Prototheca zopfii: Kruger strain UMK-B growth on acetate or N alkanes. Appl. Environ. Microbiol. 45: 333-336, 1983.

Walker JD, et al. Petroleum-degrading achlorophyllous algae Prototheca zopfii. Nature 254: 423-424, 1975.

Ueno R, et al. Optimization of heterotrophic culture conditions for n-alkane utilization and phylogenetic position based on the 18S rDNA sequence of a thermotolerant Prototheca zopfii strain. J. Biosci. Bioeng. 94: 160-165, 2002.