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Quantitative Genomic DNA from Mycoplasma pneumoniae strain M129-B7

29342DQ

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Quantitative genomic DNA from Mycoplasma pneumoniae strain M129-B7 can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
Product category
Bacteria
Product type
Nucleic acid
Molecular standard
Derived from
M129-B7 (ATCC 29342)
Organism
Mycoplasma pneumoniae Somerson et al.
Type strain
No
Genome sequenced strain
Yes
Applications
Assay development
Infectious disease research
Next-generation sequencing
Respiratory disease research
Specification range
≥1 x 105 copies/μL
Volume
100 μL
Product format
Frozen
Storage conditions
-20°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.

Characteristics

Comments
Genomic DNA isolated from a preparation of Mycoplasma pneumoniae strain M129-B7 (ATCC® 29342™). The source organism is also available through the ATCC catalog.

Handling information

Handling procedure
  1. Thaw the vial at room temperature and immediately place on ice. Avoid exposing the DNA to repeated freeze-thaw cycles as it may result in degradation.
  2. Gently mix the sample to ensure an even distribution of material.
  3. Briefly centrifuge the tube before opening to ensure all liquid is at the bottom.
Handling notes
Aliquoting is highly recommended to avoid multiple freeze-thaws.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
ATCC
Cross references
GenBank L15233 Mycoplasma pneumoniae Thr-tRNA gene.
GenBank L15236 Mycoplasma pneumoniae Arg-tRNA gene.
GenBank L15237 Mycoplasma pneumoniae Ser-tRNA gene.
GenBank L15239 Mycoplasma pneumoniae His-tRNA gene.
GenBank M32340 M.pneumoniae Trp-tRNA-UGA gene.
GenBank L15232 Mycoplasma pneumoniae Arg-tRNA gene.
GenBank L15241 Mycoplasma pneumoniae Arg-tRNA gene.
GenBank AE000019 Mycoplasma pneumoniae M129 section 61 of 63 of the complete genome.
GenBank AE000020 Mycoplasma pneumoniae M129 section 60 of 63 of the complete genome.
GenBank AE000021 Mycoplasma pneumoniae M129 section 59 of 63 of the complete genome.
GenBank AE000022 Mycoplasma pneumoniae M129 section 58 of 63 of the complete genome.
GenBank AE000023 Mycoplasma pneumoniae M129 section 57 of 63 of the complete genome.
GenBank AE000024 Mycoplasma pneumoniae M129 section 56 of 63 of the complete genome.
GenBank AE000025 Mycoplasma pneumoniae M129 section 55 of 63 of the complete genome.
GenBank AE000026 Mycoplasma pneumoniae M129 section 54 of 63 of the complete genome.
GenBank AE000027 Mycoplasma pneumoniae M129 section 53 of 63 of the complete genome.
GenBank AE000028 Mycoplasma pneumoniae M129 section 52 of 63 of the complete genome.
GenBank AE000029 Mycoplasma pneumoniae M129 section 51 of 63 of the complete genome.
GenBank AE000030 Mycoplasma pneumoniae M129 section 50 of 63 of the complete genome.
GenBank AE000031 Mycoplasma pneumoniae M129 section 49 of 63 of the complete genome.
GenBank AE000032 Mycoplasma pneumoniae M129 section 48 of 63 of the complete genome.
GenBank AE000033 Mycoplasma pneumoniae M129 section 47 of 63 of the complete genome.
GenBank AE000034 Mycoplasma pneumoniae M129 section 46 of 63 of the complete genome.
GenBank AE000035 Mycoplasma pneumoniae M129 section 45 of 63 of the complete genome.
GenBank AE000036 Mycoplasma pneumoniae M129 section 44 of 63 of the complete genome.
GenBank AE000037 Mycoplasma pneumoniae M129 section 43 of 63 of the complete genome.
GenBank AE000038 Mycoplasma pneumoniae M129 section 42 of 63 of the complete genome.
GenBank AE000039 Mycoplasma pneumoniae M129 section 41 of 63 of the complete genome.
GenBank AE000040 Mycoplasma pneumoniae M129 section 40 of 63 of the complete genome.
GenBank L15235 Mycoplasma pneumoniae Ser-tRNA genes.
GenBank AX111364 Sequence 2097 from Patent WO0123604.
GenBank X53555 M.pneumoniae gyrase genes gyrB and gyrA.
GenBank NC_000912 Mycoplasma pneumoniae, complete genome.
GenBank U00089 Mycoplasma pneumoniae M129 complete genome.
GenBank L15238 Mycoplasma pneumoniae Ile-tRNA and Ala-tRNA genes.
GenBank L15242 Mycoplasma pneumoniae Trp-tRNA and Gly-tRNA genes.
GenBank AE000008 Mycoplasma pneumoniae M129 section 9 of 63 of the complete genome.
GenBank AE000009 Mycoplasma pneumoniae M129 section 8 of 63 of the complete genome.
GenBank AE000010 Mycoplasma pneumoniae M129 section 7 of 63 of the complete genome.
GenBank AE000011 Mycoplasma pneumoniae M129 section 6 of 63 of the complete genome.
GenBank AE000012 Mycoplasma pneumoniae M129 section 5 of 63 of the complete genome.
GenBank AE000013 Mycoplasma pneumoniae M129 section 4 of 63 of the complete genome.
GenBank AE000014 Mycoplasma pneumoniae M129 section 3 of 63 of the complete genome.
GenBank AE000015 Mycoplasma pneumoniae M129 section 2 of 63 of the complete genome.
GenBank AE000016 Mycoplasma pneumoniae M129 section 1 of 63 of the complete genome.
GenBank AE000001 Mycoplasma pneumoniae M129 section 16 of 63 of the complete genome.
GenBank AE000002 Mycoplasma pneumoniae M129 section 15 of 63 of the complete genome.
GenBank AE000003 Mycoplasma pneumoniae M129 section 14 of 63 of the complete genome.
GenBank AE000004 Mycoplasma pneumoniae M129 section 13 of 63 of the complete genome.
GenBank AE000005 Mycoplasma pneumoniae M129 section 12 of 63 of the complete genome.
GenBank AE000006 Mycoplasma pneumoniae M129 section 11 of 63 of the complete genome.
GenBank AE000007 Mycoplasma pneumoniae M129 section 10 of 63 of the complete genome.
GenBank AE000017 Mycoplasma pneumoniae M129 section 63 of 63 of the complete genome.
GenBank AE000018 Mycoplasma pneumoniae M129 section 62 of 63 of the complete genome.
GenBank AE000041 Mycoplasma pneumoniae M129 section 39 of 63 of the complete genome.
GenBank AE000042 Mycoplasma pneumoniae M129 section 38 of 63 of the complete genome.
GenBank AE000043 Mycoplasma pneumoniae M129 section 37 of 63 of the complete genome.
GenBank AE000044 Mycoplasma pneumoniae M129 section 36 of 63 of the complete genome.
GenBank AE000045 Mycoplasma pneumoniae M129 section 35 of 63 of the complete genome.
GenBank AE000046 Mycoplasma pneumoniae M129 section 34 of 63 of the complete genome.
GenBank AE000047 Mycoplasma pneumoniae M129 section 33 of 63 of the complete genome.
GenBank AE000048 Mycoplasma pneumoniae M129 section 32 of 63 of the complete genome.
GenBank AE000049 Mycoplasma pneumoniae M129 section 31 of 63 of the complete genome.
GenBank AE000050 Mycoplasma pneumoniae M129 section 30 of 63 of the complete genome.
GenBank AE000051 Mycoplasma pneumoniae M129 section 29 of 63 of the complete genome.
GenBank AE000052 Mycoplasma pneumoniae M129 section 28 of 63 of the complete genome.
GenBank AE000053 Mycoplasma pneumoniae M129 section 27 of 63 of the complete genome.
GenBank AE000054 Mycoplasma pneumoniae M129 section 26 of 63 of the complete genome.
GenBank AE000055 Mycoplasma pneumoniae M129 section 25 of 63 of the complete genome.
GenBank AE000056 Mycoplasma pneumoniae M129 section 24 of 63 of the complete genome.
GenBank AE000057 Mycoplasma pneumoniae M129 section 23 of 63 of the complete genome.
GenBank AE000058 Mycoplasma pneumoniae M129 section 22 of 63 of the complete genome.
GenBank AE000059 Mycoplasma pneumoniae M129 section 21 of 63 of the complete genome.
GenBank AE000060 Mycoplasma pneumoniae M129 section 20 of 63 of the complete genome.
GenBank AE000061 Mycoplasma pneumoniae M129 section 19 of 63 of the complete genome.
GenBank AE000062 Mycoplasma pneumoniae M129 section 18 of 63 of the complete genome.
GenBank AE000063 Mycoplasma pneumoniae M129 section 17 of 63 of the complete genome.
GenBank M21519 M.pneumoniae P1 operon encoding P1 surface protein, ORF-4, and ORF-6 proteins, complete cds.
GenBank L15240 Mycoplasma pneumoniae Asn-tRNA, Glu-tRNA, Thr-tRNA, Val-tRNA, Thr-tRNA, Lys-tRNA and Leu-tRNA genes.
GenBank L15234 Mycoplasma pneumoniae Cys-tRNA, Pro-tRNA, Met-tRNA, Ile-tRNA, Ser-tRNA, fMet-tRNA, Asp-tRNA and Phe-tRNA genes.
GenBank AF132741 Mycoplasma pneumoniae strain ATCC 29342 16S ribosomal RNA gene and 16S-23S rRNA intergenic spacer, complete sequence.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Lipman RP, et al. Characteristics of virulent, attenuated, and avirulent Mycoplasma pneumoniae strains. J. Bacteriol. 100: 1037-1043, 1969. PubMed: 5359607

Lipman RP, Clyde WA Jr.. The interrelationship of virulence, cytadsorption, and peroxide formation in Mycoplasma pneumoniae. Proc. Soc. Exp. Biol. Med. 131: 1163-1167, 1969. PubMed: 5811969

Collier AM, et al. Biologic effects of Mycoplasma pneumoniae and other mycoplasmas from man on hamster tracheal organ culture. Proc. Soc. Exp. Biol. Med. 132: 1153-1158, 1969. PubMed: 5391202

Krause DC, et al. Transposon mutagenesis reinforces the correlation between Mycoplasma pneumoniae cytoskeletal protein HMW2 and cytadherence. J. Bacteriol. 179: 2668-2677, 1997. PubMed: 9098066

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