Saccharomyces cerevisiae Meyen ex E.C. Hansen
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the test tube with sterile distilled water. Mix well.
4. Let the test tube sit for at least 2 hours.
5. Mix the suspension well. Use several drops to inoculate a test tube of slant or a plate with recommended medium.
6. Incubate the test tube or plate at the temperature recommended.
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
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Sharma CB, et al. N-Glycosylation of yeast proteins. Characterization of the solubilized oligosaccharyl transferase. Eur. J. Biochem. 116: 101-108, 1981. PubMed: 7018901
Ramirez M, et al. A similar protein portion for two exoglucanases secreted by Saccharomyces cerevisiae. Arch. Microbiol. 151: 391-398, 1989. PubMed: 2500920
Sinclair K, et al. The ASP1 gene of Saccharomyces cerevisiae, encoding the intracellular isozyme of L-asparaginase. Gene 144: 37-43, 1994. PubMed: 8026756
Nieto A, et al. Cloning and characterization of the SEC18 gene from Candida albicans. Yeast 9: 875-887, 1993. PubMed: 8212895
. Physiology of immobilized cells. Amsterdam: Elsevier; 1990.