Expanded Primary Hepatocytes: Achieve More Predictive Toxicity Studies

1/30/2020


Abstract

Choosing the ideal model for toxicological research and screening poses a challenge to the researcher. On one hand, you want the high biological relevance of a primary cell so you can trust that your assay is predictive of the in vivo situation. On the other, you need the proliferative capacity of a cell line to provide a copious quantity of cells so you can test all of your variable conditions. ATCC upcyte® Hepatocytes provide both qualities. This advanced hepatic model exploits a growth method that directs non-dividing primary cells to proliferate without altering their biological properties, allowing for the generation of large batches of homogeneous yet physiologically relevant hepatocytes. This webinar will provide an overview of upcyte® Hepatocytes, highlighting the various uses for the cells, such as hepatoxicity prediction and drug-drug interaction studies.

Key Points:

  • Toxicologists need the high biological relevance of primary cells and the proliferative capacity of cell lines for standard, predictive assays; neither model provides both characteristics.
  • upcyte® technology offers sufficient quantities of hepatocytes that exhibit primary cell physiologies such as CYP activity. Long-term culturing is possible to detect low level hepatotoxicity.
  • upcyte® Hepatocytes are fully characterized with validated performance data, and they are tested for CYP induction and inhibition.

Presenters

Kevin Grady

Kevin Grady, B.S.,
Senior Product Line Business Manager, ATCC

Kevin Grady is the Senior Product Line Business Manager for Cell Biology at ATCC. He has been with ATCC for 5 years; prior to ATCC, he held positions at Lonza as Worldwide Product Manager and Director of Scientific Support. Kevin has a long history in the life science industry additionally serving as Director of Scientific Support at Amaxa and Manager of Technical Support at Life Technologies. Mr. Grady has always found great satisfaction in helping researchers learn how to use available products and tools to be more productive and successful in reaching their research goals.