American Type Culture Collection (ATCC) Logo American Type Culture Collection (ATCC) Logo 0
  • Quick Order
  • Careers
  • Support

Prevotella melaninogenica (Oliver and Wherry) Shah and Collins emend. Wu et al.

25845

Download Genome Learn about the ATCC Genome Portal
Prevotella melaninogenica strain VPI 2381 [B282, JCM 6325, VPI 4196] was isolated from sputum. This whole-genome sequenced bacterial type strain can be used in media testing.
Product category
Bacteria
Product type
Anaerobe
Preceptrol culture
Strain designation
VPI 2381 [B282, JCM 6325, VPI 4196]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Sputum; Dept. of Health and Environmental Control
Geographical isolation
United States; South Carolina
Applications
Bioinformatics
Media testing
Product format
Frozen
Storage conditions
-80°C or colder
Buy Now
Price: $84.00 EA
Discounts may be available for our fellow nonprofit organizations. Login to see your price.

Generally ships within 1-3 business days

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Media testing
Preceptrol
Yes

Characteristics

Comments
Genome sequencing strain

Handling information

Medium
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
  1. Open vial according to enclosed instructions.                            
  2. Under anaerobic conditions, withdraw 0.5 mL of #2863 from a single test tube (5 to 6 mL) and rehydrate the entire vial contents.   
  3. Aseptically transfer this aliquot back into the broth tube. A brucella blood plate and a secondary broth maybe inoculated.  An aerobic blood plate may also be streaked to check for purity.
  4. Incubate tubes and one plate under anaerobic conditions at 37°C.  Incubate one blood plate aerobically at 37°C.
  5. Within 24 to 48 hours, growth is evident by turbidity in the broth and colonies on an agar plate.  No growth should occur on the blood agar plate incubated aerobically.

 

ANAEROBIC CONDITIONS:   
Anaerobic conditions for transfer may be obtained by either of the following:

  • Use of an anaerobic gas chamber, or                                        
  • Placement of test tubes under a gassing cannula system connected to anaerobic gas.   

 

Anaerobic conditions for incubation may be obtained by any of the following:

  • Loose screw caps on test tubes in anaerobic chamber,                       
  • Loose screw caps on test tubes in an activated anaerobic gas pack jar, or   
  • Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.   
Handling notes
ATCC recommends Anaerobe Systems PRAS Brucella Blood Plate (AS-111 or AS-141). 

If grown on Anaerobic Brain Heart Infusion agar with 5% Laked (hemolyzed) Blood and supplemented with Vitamin K and Hemin, the black pigment production will be stronger and seen earlier. 

Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions.  Resazurin in the media is a color indicator for anaerobic conditions.  Observance of a pink color in medium before use, or during incubation, shows anaerobic conditions have not been met and oxidation has occurred.  Medium should be discarded.

Purified genomic DNA of this strain is available as ATCC® 25845D-5™.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Bacteroides melaninogenicus subsp. melaninogenicus (Oliver and Wherry) Holdeman and Moore
Depositors
LV Holdeman
Chain of custody
ATCC <-- LV Holdeman <-- S Finegold B282
Cross references
GenBank AX110258 Sequence 991 from Patent WO0123604.
GenBank L16469 Prevotella melaninogenica ATCC 25845 16S ribosomal RNA gene, complete sequence.
GenBank ACSI01000000 Prevotella melaninogenica ATCC 25845, whole genome shotgun sequencing project.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Holdeman LV, Johnson JL. Description of Bacteroides loescheii sp. nov. and emendation of the descriptions of Bacteroides melaninogenicus (Oliver and Wherry) Roy and Kelly 1939 and Bacteroides denticola Shah and Collins 1981. Int. J. Syst. Bacteriol. 32: 399-409, 1982.

Shah HN, Collins DM. Prevotella, a new genus to include Bacteroides melaninogenicus and related species formerly classified in the genus Bacteroides. Int. J. Syst. Bacteriol. 40: 205-208, 1990. PubMed: 2223612

Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Wu CC, et al. Emended descriptions of Prevotella denticola, Prevotella loescheii, Prevotella veroralis, and Prevotella melaninogenica. Int. J. Syst. Bacteriol. 42: 536-541, 1992.

View All Curated Citations for this Product

For product-related inquiries and issues, contact Technical Service:

Message Us

Hours of Operation

Monday - Friday
9:00am - 5:00pm
US Eastern Time