Butyricicoccus porcorum (TSD-102)

Strain Designations: BB10  /  Biosafety Level: 1

Permits And Restrictions

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Strain Designations BB10
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Depositor Recommended Growth Conditions Grow strictly anaerobically (recommend 86% N2, 10% CO2, 4% H2) in BB`10 General Growth Media at 39°C for 2 days.

BB10 General Growth Media
5.0 g Tryptone peptone
5.0 g Yeast extract
1 mL Resazurin (0.1% w/vol)
40 mL Salt solution 1
40 mL Salt solution 2

1 mL Hemin solution
1 mL Vitamin K solution, working stock

0.5 g Cysteine-HCl

2.5 g Glucose

2.5 g Galactose

2.5 g Lactose
2.5 g Maltose
4.5 g Sodium Acetate
dH2O up to 1 L

Salt solution 1:
6 g K2HPO4
dH2O up to 1 L

Salt solution 2 (per liter):

6 g   KH2PO4

12 g  (NH4)2SO4

12 g  NaCl

1.2 g  MgSO4·7H2O

1.2 g  CaCl·6H2O

Hemin solution:
0.5g Hemin into 10 mL 1M NaOH. 
Bring up to 100 mL in dH2O. 
Store at 4°C.

Vitamin K solution:
Stock: 0.02 mL vitamin K into 2 mL 95% ethanol. Store in dark at 4°C.
Working stock: add 1 mL stock to 9 mL d H2O. Use within 3 days.
Name of Depositor HK Allen, United States Department of Agriculture - Agriculture Research Service, Beltsville, Maryland, USA.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation