Antigen expression: This cell line is positive for epithelial marker pan-cytokeratin (immunocytochemistry)(verified at ATCC), positive for epithelial cell adhesion molecule E-cadherin (immunocytochemistry) (verified at ATCC),
The RPTEC/TERT1 cells express both Aminopeptidase N (verified at ATCC) and γ-Glutamyl Transferase (GGT) that are located in the brush border of the renal proximal tubular epithelium.
The RPTEC/TERT1 cells specifically respond to parathyroid hormone (PTH) but not arginine vasopressin (AVP), and react with enhanced ammonia genesis on lowering of the environmental pH.
The RPTEC/TERT1 cells exhibit sodium-dependent uptake of phosphate as well as intact functionality of the megalin/cubilin transport system.
RPTEC/TERT1 cells show the characteristic morphology and functional properties of normal proximal tubular epithelial cells.
At high cell densities, the RPTEC/TERT1 cells form characteristic "domes", maintain ultrastructural organization with tight junctions, densely packed microvilli and primary cilium, indicating functional cell polarization.
When cultured on the Corning™ Transwell™ Permeable membrane cell culture insert, the RPTEC/TERT1 cells at confluence form intact functional barrier as indicated by stabilized Trans-Epithelial Electrical Resistance (TEER) across the membrane.