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Vector Information
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Size (kb): 5.6799998283386230 Vector: pTSEC (plasmid) Promoters: Promoter Ptrc Construction: pSTR4-2 Marker(s):ampR Construct size (kb): 5.679999828338623 Features: primer site: 5'-TTGACAATTAATCATCCGGC-3'
promoter: Ptrc
terminator: rrnBT1
terminator: rrnBT2
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--uncut; EcoRI--5.6; PstI--5.6; EcoRI/PstI--4.4, 1.3. Overproduces yeast cystathionine beta-synthase (truncated).
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References
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Jhee KH, et al. Domain architecture of the heme-independent yeast cystathionine beta-synthase provides insights into mechanisms of catalysis and regulation. Biochemistry 39: 10548-10556, 2000. PubMed: 10956046
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Shipping Information
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Frozen glycerol stock of E. coli containing the plasmid.
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