S. cerevisiae/E. coli marker swap vectors

To convert the host phenotype from the existing yeast auxotrophic marker to a new marker (eg. HIS3 → LEU2), transform with the restriction enzyme digested vector (eg. ApaI+PstI digested pHL3) and select for the appropriate phenotype (for pHL3, Leu+). 

Some combinations of marker swap plasmids and target locus may result in relatively high reversion rates.  In most, but not all cases, the frequencies of successful convertants are greater than 30%.  When swapping markers on an episomal plasmid, appropriate phenotype may result from loss of the plasmid unless a second selectable or scorable marker is used to ensure plasmid maintenance.                      

Refer to reference for more information.                                                                                  

- Yeast 13: 647-653, 1997

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