HTLV-II, deltaH6H11, in pBR322, purified plasmid DNA
45134D ™
Full length clone of Human T-cell leukemia virus II.
Constructed by inserting the HindIII fragment from lambdaH6 into the HindIII site of pBR322 that had been modified by an EcoRV-MscI deletion of approximately 1.25 kb.
The full-length plasmid is unstable in bacterial hosts, and is distributed as purified plasmid DNA. The insert contains the following restriction sites (approximate kb from 5' end): EcoRI--0.1, 0.8, 4.7, 9.0; KpnI--1.7, 3.4; NheI--1.0, 6.65; SacI--0.5, 4.0, 8.7; XbaI--3.6; BamHI--0.3, 5.0, 8.4.
- personal communication
The viral portion of the insert is 8.8 kb. The insert also includes cellular sequences derived from Mo (T cell leukemia) cells.
- Nature 305: 502-505, 1983
Size of Construct (kb): 13.2