Hamamotoa singularis (Phaff et Carmo-Sousa) Wang et al. (ATCC® 24193)

Strain Designations: UCD 60-79 [CBS 5109, DBVPG 6620, JCM 5356, NCYC 900, NRRL Y-5852]  /  Product Format: freeze-dried

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Deposited As Sporobolomyces singularis Phaff et do Carmo-Sousa
Strain Designations UCD 60-79 [CBS 5109, DBVPG 6620, JCM 5356, NCYC 900, NRRL Y-5852]
Application
produces arabinosylcytosine derivatives
produces pyridoxine derivatives
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain yes
Preceptrol® no
Morphology

After 5 days at 25°C colonies are mucoid, smooth, shiny, pale yellowish-brown.  Cells are ellipsoidal to subglobose, 6.0-12.0 x 2.5-6.0 um, and single.  Budding is polar. 

 

Sequenced Data
No DNA sequencing was performed in house on this product.
Morphology

After 5 days at 25°C colonies are mucoid, smooth, shiny, pale yellowish-brown.  Cells are ellipsoidal to subglobose, 6.0-12.0 x 2.5-6.0 um, and single.  Budding is polar. 

 

Name of Depositor HJ Phaff
Isolation
Frass of Scolytus tsugae in Tsuga heterophylla, Oregon
Cross References

Nucleotide (GenBank) : AB021690 gene for 18S rRNA

Nucleotide (GenBank) : AF189996 26S ribosomal RNA gene, partial sequence

References

Phaff HJ, do Carmo-Sousa L. Four new species of yeast iosolated from insect frass in bark of Tsuga heterophylla (Raf.) Sargent. Antonie van Leeuwenhoek 28: 193-207, 1962.

Suzuki Y, Uchida K. Formation of beta-galactosyl compounds of pyridoxine in growing culture of Sporobolomyces singularis. Biochim. Biophys. Acta 1116: 67-71, 1992. PubMed: 1540625

Suzuki Y, Uchida K. Formation of beta-galactosyl compounds of arabinosylcytosine in growing culture of Sporobolomyces singularis. Biosci. Biotechnol. Biochem. 58: 639-643, 1994. PubMed: 7764857

Fell JW, et al. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int. J. Syst. Evol. Microbiol. 50: 1351-1371, 2000. PubMed: 10843082

Hamamoto M, Nakase T. Phylogenetic analysis of the ballistoconidium-forming yeast genus Sporobolomyces based on 18S rDNA sequences. Int. J. Syst. Evol. Microbiol. 50: 1373-1380, 2000. PubMed: 10843083

Wang QM, et al. Phylogenetic classification of yeasts and related taxa within Pucciniomycotina. Stud Mycol 81: 149-189, 2015. PubMed: 26951631

Scorzetti G, et al. Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res. 2: 495–517, 2002. PubMed: 12702266

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation