Clostridium difficile (Hall and O'Toole) Prevot (ATCC® BAA-1382-FZ)

Strain Designations: 630  /  Type Strain: no  /  Biosafety Level: 2

Strain Designations 630
Isolation
Clinical isolate
Switzerland
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Shipping Information Frozen
Preceptrol® no
Genotype Toxinotype: 0
Type Strain no
Comments
Preparation of Clostridium difficile strain 630 ATCC® BAA-1382-FZ™, frozen for ease of use. Lot specific titers are available upon request.
Toxinotype 0
Ribotype 012
Presence of tcdA and tcdB genes confirmed by PCR.
Binary toxin gene cdtB not amplified by PCR
Genome sequenced strain
Medium ATCC® Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic gas mixture 80% N2 -10% CO2 -10% H2
Genotype Toxinotype: 0
Name of Depositor B Wren, H Maschler
Isolation
Clinical isolate
Switzerland
References

Wust Jurg, et al. Investigation of an Outbreak of Antibiotic-Associated Colitis by Various Typing Methods. J. Clin. Microbiol. 16: 1096-1101, 1982.

Sebaihia Mohammed. The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome. Nat. Genet. 38: 779-786, 2006.

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986