Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Size (kb): 8.37
Vector: pCM189 (plasmid)
Promoters: Promoter CMV
Construct size (kb): 8.37
Features: marker(s): URA3
operator: tetO - seven repeats
other: tTA transcriptional activator
YC-type (centromeric) shuttle vector
Restriction digests of the clone give the following sizes (kb): BamHI--8.4; PstI--8.4; BamHI/XhoI--7.7, 0.67.
Yeast centromeric expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656
- 87662) differing in the level of expression, mode of replication and selectable marker.
Gari E, et al. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast 13: 837-848, 1997. PubMed: 9234672