YEpFAT6 (ATCC® 87644)

Applications: M9 + leucine + tryptophan medium recommended for DNA purification.  /  Depositors: K Runge

Permits and Restrictions

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Designations YEpFAT6
Depositors K Runge
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli MC1066
Vector Information
Size (kb): 10.6840000152587900
DESCRIPTION OF VECTOR:
Intact vector size: 10.684
Type of vector: plasmid
Cloning sites: SacI SmaI SalI
Polylinker sites: EcoRI SacI AvaI KpnI SmaI BamHI XbaI SalI PstI HindIII
Other unique sites: NotI
Host range: Schizosaccharomyces pombe; Saccharomyces cerevisiae;
Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: T7, ->, 9382-9401
MCS: EcoRI...HindIII, ->, 10635-2
marker(s): GART, <-, 2-2091
replicon: 2 micron/LEU2-d, 2086-4780
marker(s): LEU2-d, ->, 3443-4537
marker(s): URA4, <-, 4928-5722
promoter for in vitro transcription: T3, <-, 6261-6280
promoter: lac, <-, 6350-6378
replicon: pMB1, 6704-6704
marker(s): ampR, <-, 7462-8322
Vector: YEpFAT6 (plasmid)
Promoters: Promoter for in vitro transcription T7
Marker(s):ampR,LEU2-d,ura4+
Construct size (kb): 10.68400001525879
Features: marker(s): GART
marker(s): LEU2-d
marker(s): URA4
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron/LEU2-d
replicon: pMB1
MCS: EcoRI...HindIII
Applications
M9 + leucine + tryptophan medium recommended for DNA purification.
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--10.0. 1.0; SmaI--11.0; HindIII--6.55, 4.8.
M9 + leucine + tryptophan medium recommended for DNA purification.
One of 9 YE-type shuttle vectors (ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Runge KW, Zakian VA. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol. Cell. Biol. 9: 1488-1497, 1989. PubMed: 2657397

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map