Host
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Distribution host: Escherichia coli MC1066
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Vector Information
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Size (kb): 10.6840000152587900 DESCRIPTION OF VECTOR: Intact vector size: 10.684 Type of vector: plasmid Cloning sites: SacI SmaI SalI Polylinker sites: EcoRI SacI AvaI KpnI SmaI BamHI XbaI SalI PstI HindIII Other unique sites: NotI Host range: Schizosaccharomyces pombe; Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): promoter for in vitro transcription: T7, ->, 9382-9401 MCS: EcoRI...HindIII, ->, 10635-2 marker(s): GART, <-, 2-2091 replicon: 2 micron/LEU2-d, 2086-4780 marker(s): LEU2-d, ->, 3443-4537 marker(s): URA4, <-, 4928-5722 promoter for in vitro transcription: T3, <-, 6261-6280 promoter: lac, <-, 6350-6378 replicon: pMB1, 6704-6704 marker(s): ampR, <-, 7462-8322 Vector: YEpFAT6 (plasmid) Promoters: Promoter for in vitro transcription T7 Marker(s):ampR,LEU2-d,ura4+ Construct size (kb): 10.68400001525879 Features: marker(s): GART marker(s): LEU2-d marker(s): URA4 marker(s): ampR promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: 2 micron/LEU2-d replicon: pMB1 MCS: EcoRI...HindIII
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Applications
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M9 + leucine + tryptophan medium recommended for DNA purification.
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--10.0. 1.0; SmaI--11.0; HindIII--6.55, 4.8. M9 + leucine + tryptophan medium recommended for DNA purification. One of 9 YE-type shuttle vectors ( ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine.
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References
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Runge KW, Zakian VA. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol. Cell. Biol. 9: 1488-1497, 1989. PubMed: 2657397
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Shipping Information
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Distributed: freeze-dried
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