YEplac181 (ATCC® 87588)

Applications: YE-type (episomal) shuttle vectorvector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: RD Gietz

Permits and Restrictions

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Designations YEplac181
Depositors RD Gietz
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.7410001754760740
Vector: YEplac181 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):LEU2,ampR
Construct size (kb): 5.741000175476074
Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: lac
replicon: 2 micron ori
replicon: pMB1
MCS: HindIII...EcoRI
Applications
YE-type (episomal) shuttle vector
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): AvaI--5.2, 0.8; ClaI--6.0; EcoRI--6.0.
One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1).
The EcoRI and KpnI restriction sites from the LEU2 gene of S. cerevisiae were removed by using oligo-directed mutagenesis.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106

Related Products
component of:ATCC 87623
component of:ATCC 87638
Shipped frozen
Shipping Information

 Freeze dried Escherichia coli containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87588