pOSEX2 (ATCC® 87211)

Applications: expression vector  /  Depositors: E Bremer

Designations pOSEX2
Permits and Restrictions

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Depositors E Bremer
Biosafety Level 1
Distribution host: Escherichia coli MKH13 (ATCC 47087)
Vector Information
Size (kb): 5.3800001144409180
Intact vector size: 5.380
Type of vector: plasmid
Cloning sites: BamHI mLuI BfrI NotI EclXI KspI Asp718 KpnI ApaI SalI XbaI
SacI SphI EcoRV NcoI HindIII
Polylinker sites: BamHI mLuI BfrI NotI EclXI KspI Asp718 KpnI ApaI SalI XbaI
SacI PstI SphI EcoRV NcoI StyI HindIII
Construction: pUC-BM20, proU (pJL32-3), rrnB (pTrc99A), pBR322
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR, ->, 84-1274
replicon: rop (copy number control), 1913-2104
replicon: pMB1, 2532-2532
marker(s): ampR, <-, 3291-4161
transcription terminator: rrnB T2, <-, 4263-4340
transcription terminator: rrnB T1, <-, 4422-4465
MCS: BamHI...HindIII, <-, 4749-4672
coding sequence: proV 5' sequence, <-, 4756-5065
promoter for expression: proU, <-, 5063-5366
Vector: pOSEX2 (plasmid)
Promoters: Promoter for expression proU
Construction: pUC-BM20, proU (pJL32-3), rrnB (pTrc99A), pBR322
Construct size (kb): 5.380000114440918
Features: marker(s): ampR
marker(s): tetR
promoter for expression: proU
replicon: pMB1
replicon: rop (copy number control)
MCS: BamHI...HindIII
transcription terminator: rrnB T1
transcription terminator: rrnB T2
coding sequence: proV 5' sequence
expression vector
Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 1.05; HindIII--4.6, 0.8; PvuII--5.4.
Expression can be induced in cells grown in low osmolarity media by the addition of sodium chloride.
Vector does not provide a ribosome binding site or initiation codon.
Expression vector allowing osmotically controlled expression of cloned inserts directed by the E. coli proU promoter.
This material is being provided with the explicit understanding that it not be used for commercial purposes without prior authorization from the depositor.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Herbst B, et al. pOSEX: vectors for osmotically controlled and finely tuned gene expression in Escherichia coli. Gene 151: 137-142, 1994. PubMed: 7828862

Erhard Bremer, personal communication

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