pLDR23 [WM2196] (ATCC® 87208)

Applications: contains easily purifiable cassette(s) for construction gene 10 promoter cassette  /  Depositors: W Messer

Permits and Restrictions

View Permits

Designations pLDR23 [WM2196]
Depositors W Messer
Biosafety Level 1
Vector Information
Size (kb): 2.4530000686645510
Vector: pLDR23 (plasmid)
Promoters: Promoter T7 (phi10)
Construction: pT7-7
Marker(s):ampR
Construct size (kb): 2.453000068664551
Features: E. coli promoter cassette: T7 phi10 promoter cassette
marker(s): ampR
promoter: T7 (phi10)
replicon: pMB1
Applications
contains easily purifiable cassette(s) for construction gene 10 promoter cassette
Comments
Restriction digests of the clone give the following sizes (kb): BglI--2.5; ClaI--2.5; XhoI--2.1, 0.3.
Helper plasmid with an XhoI promoter cassette containing the T7 phi10 promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map