pLDR23 [WM2196] (ATCC® 87208)

Applications: contains easily purifiable cassette(s) for construction gene 10 promoter cassette  /  Depositors: W Messer

Permits and Restrictions

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Designations pLDR23 [WM2196]
Depositors W Messer
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 2.4530000686645510
Vector: pLDR23 (plasmid)
Promoters: Promoter T7 (phi10)
Construction: pT7-7
Marker(s):ampR
Construct size (kb): 2.453000068664551
Features: E. coli promoter cassette: T7 phi10 promoter cassette
marker(s): ampR
promoter: T7 (phi10)
replicon: pMB1
Applications
contains easily purifiable cassette(s) for construction gene 10 promoter cassette
Comments
Restriction digests of the clone give the following sizes (kb): BglI--2.5; ClaI--2.5; XhoI--2.1, 0.3.
Helper plasmid with an XhoI promoter cassette containing the T7 phi10 promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map