Size (kb): 4.1440000534057620
Vector: pBSL159 (plasmid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construct size (kb): 4.144000053405762
Features: marker(s): cmlR
promoter for in vitro transcription: T7 (phi10)
MCS: KpnI...SacI MluI
MCS: MluI SacI...KpnI
coding sequence: bla (ampR)
contains easily purifiable cassette(s) for construction omega - Ap
Restriction digests of the clone give the following sizes (kb): BamHI--2.1, 2.0; EcoRI--2.1 (doublet); KpnI--2.15, 2.0; MluI--2.2, 2.0; SstI--2.15, 2.0.
Plasmid useful for generation of marked deletions and insertions in cloned DNA in vitro. Contains an omega - Ap element flanked by multiple cloning sites duplicated in inverted orientation.
The PstI and HincII sites in the bla gene have been removed.