Distribution host: Escherichia coli JM101
Size (kb): 5.0999999046325680
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pMR102
Intact vector size: 4.100
Type of vector: phagemid
Vector end: SalI
Vector end: PstI
Cloning sites: NcoI BamHI
Other unique sites: EcoNI, SalI, BstEII, PstI
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: M13, ->, 11-467
replicon: p15A, ->, 1585-1588
marker(s): kanR, ->, 2179-2994
operator: lac, <-, 3784-3800
promoter for expression: T7 (phi10), <-, 3804-3823
Vector: pMR103 (phagemid)
Promoters: Promoter for expression T7 (phi10)
Construction: pMR102, pET11d
Construct size (kb): 5.099999904632568
Features: marker(s): kanR
promoter for expression: T7 (phi10)
repressor gene: lacI
vector permitting production of single-stranded DNA
Restriction digests of the clone give the following sizes (kb): PstI/SalI--5.2; PstI--5.2; SalI--uncut; EcoRV--3.2, 2.0; BstEII--3.6, 1.6; BstEII/PstI--3.0, 1.7, 0.5.
The SalI site was lost during cloning.
The lacI gene permits tight regulation of transcription of an insert and synthesis of potentially toxic proteins.
Expression vector (T7-based) with a kanR marker and a P15A replicon compatible with ColE1-derived plasmids. Particularly useful for co-transformation with ColE1-based ampR T7 expression vectors and the production of two proteins in the same cell.
If used in an Escherichia coli strain that expresses T7 polymerase under the control of the lacUV5 promotor (such as BL21(DE3)), addition of IPTG can result in high levels of recombinant protein production.
Munson M, et al. ColE1-compatible vectors for high-level expression of cloned DNAs from the T7 promoter. Gene 144: 59-62, 1994. PubMed: 8026759