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pACYC177/ET3d/yNMT

87052

Organism
Saccharomyces cerevisiae Meyen ex E.C. Hansen
Classification
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Clone type
Vector
Applications
Molecular biology
Product format
Freeze-dried
Mission Collection Item
This is a Mission Collection Item.

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
produces protein peptide N-myristoyl transferase glycylpeptide N-tetradecanoyltransferase

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): NdeI--5.5; NcoI--5.5; StuI--3.9, 1.5.
Expression system compatible with ColE1 plasmids, for cotransformation and production of myristoylated proteins.
Introduction of the NcoI site during amplification introduced a single base substitution T->G at nt 4.
Expression of this gene in the presence of 100 uM myristic acid allows myristoylation of recombinant proteins produced by a second ColE1 plasmid.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
4.900000095367432
Vector name
pACYC177/ET3d
Type of vector
plasmid
Construction
pACYC177,pET3d
Host range
Escherichia coli
Vector end
NcoI; BamHI
Cloning sites
NcoI; NcoI+BamHI
Coding sequence
NMT1
Markers
kanR
Promoters
Expression: T7 (phi10)
Replicon
p15A
Ribosome binding site
gene 10

Insert information

Insert size (kb)
1.3999999999999999
Type of DNA
genomic
Insert information
Insert 5' end: NcoI
Insert 3' end: BglII
Cross references: DNA Seq. Acc.: M23726
Nucleotides 5-1392 of the insert correspond to
nucleotides 190-1577 of M23726.
Chromosome
XII
XII R
Target gene
peptide N-myristoyl transferase
Gene name
peptide N-myristoyl transferase
Gene product
peptide N-myristoyl transferase(glycylpeptide N-tetradecanoyltransferase) [NMT1]
Gene symbol
NMT1
Contains complete coding sequence
Yes

Handling information

Host
Escherichia coli BL21(DE3)
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): NdeI--5.5;
NcoI--5.5; StuI--3.9, 1.5. U25270, Expression cloning vector pACYC177/ET3d/yNMT,
complete sequence.
- ATCC staff

Expression system compatible with ColE1 plasmids, for cotransformation and
production of myristoylated proteins.
- J. Biol. Chem. 268: 7064-7068, 1993

Expression of this gene in the presence of 100 uM myristic acid allows
myristoylation of recombinant proteins produced by a second ColE1 plasmid.
- J. Biol. Chem. 268: 7064-7068, 1993

Introduction of the NcoI site during amplification introduced a single base
substitution T->G at nt 4.
- J. Biol. Chem. 268: 7064-7068, 1993

History

Depositors
RS Haun
Special collection
NCRR Contract
Cross references
GenBank M23726
GenBank U25270 Expression cloning vector pACYC177/ET3d/yNMT, complete sequence.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Haun RS, et al. Effect of myristoylation on GTP-dependent binding of ADP-ribosylation factor to Golgi. J. Biol. Chem. 268: 7064-7068, 1993. PubMed: 8463239

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