pXC17 (ATCC® 86991)

Applications: expression vectorin another host, produces proteinvector useful for cloning PCR products  /  Depositors: TA Patterson

Permits and Restrictions

View Permits

Designations pXC17
Depositors TA Patterson
Biosafety Level 1
Vector Information
Size (kb): 5.7589998245239260
Vector: pXC17 (plasmid)
Promoters: Promoter for expression lambda PL
Construction: pXC23, oligo cassette
Marker(s):ampR
Construct size (kb): 5.758999824523926
Features: initiation codon: ATG
marker(s): ampR
marker(s): tetR
promoter for expression: lambda PL
replicon: ROP copy number control
replicon: pMB1
restriction site: 3' sites HindIII...BamHI
restriction site: 5' ClaI site
ribosome-binding site: synthetic Shine-Dalgarno
spacer sequence: AAAAACATCG
transcription terminator: partially deleted lambda terminator
coding sequence: 14-3-3
Applications
expression vector
in another host, produces protein
vector useful for cloning PCR products
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; HindIII--5.8.
The vector contains a 0.7 kb bovine cDNA that can be excised using the 5' and 3' cloning sites and be replaced by a gene of interest. The bovine insert may be used as a positive control for expression.
One of 12 expression vectors (ATCC 86990-87001) designed to maximize expression from the lambda PL promoter and support cloning of PCR products. The vectors differ in cloning sites and in translational enhancer and initiation sequences.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Cheng X, Patterson TA. Construction and use of lambda PL promoter vectors for direct cloning and high level expression of PCR amplified DNA coding sequences. Nucleic Acids Res. 20: 4591-4598, 1992. PubMed: 1408761

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map