Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Size (kb): 4.8010001182556150
Vector: pFL59- (phagemid)
Promoters: Promoter lac
Construct size (kb): 4.801000118255615
Features: insert detection: lacZ'
marker(s): ampR, TRP1
replicon: pMB1, f1, ARS-CEN6
YC-type (centromeric) shuttle vector
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--4.8; BglII--4.0, 0.85; ClaI--4.0, 0.8.
YC-type low copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. Can be used to produce ssDNA.
The order of the major features in this plasmid is: EcoRI/MCS/HindIII - TRP1 - ARS-CEN6 - pMB1 ori - ampR - f1 ori.
Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470
Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589
Bach ML, et al. Evidence for transcriptional regulation of OMP-decarboxylase in yeast by hybridisation of mRNA to the yeast structural gene cloned in E. coli. Proc. Natl. Acad. Sci. USA 76: 386-390, 1979. PubMed: 370827
Dotto GP, et al. The functional origin of Bacteriophage f1 DNA replication. J. Mol. Biol. 172: 507-521, 1984. PubMed: 6607361
Panzeri L, Philippsen P. Centromeric DNA from chromosome VI in Saccharomyces cerevisiae. EMBO J. 1: 1605-1611, 1982. PubMed: 6327282