Size (kb): 7.1640000343322750
Vector: pCMVbeta (plasmid)
Promoters: Promoter CMV immediate early
Construction: pUC19, pC4AUG beta-gal, pCM5029, pL1
Construct size (kb): 7.164000034332275
Features: eukaryotic promoter/expression cassette: CMV promoter/enhancer SV40 splice signal
eukaryotic reporter gene cassette: lacZ
promoter: CMV immediate early
reporter group: lacZ
terminator: SV40 late
enhancer: CMV immediate early
contains easily purifiable cassette(s) for construction
vector permitting visual detection of activity in colonies
Restriction digests of the clone give the following sizes (kb): EcoRI--7.3; HindIII--7.3; EcoRI/HindIII--4.6, 2.7; NotI--3.8, 3.5; BamHI--3.65; 3.45; 0.2; NotI/EcoRI--3.54, 2.9, 0.9.
Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining.
Designed to express high levels of beta-galactosidase or an alternative gene product. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors.
The lacZ gene may be excised by NotI and replaced with alternative sequences.
The order of the major features in this plasmid is: pUC19 - EcoRI - PstI - CMV immediate early promoter/enhancer - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - PstI - HindIII - pUC19.
MacGregor GR, Caskey CT. Construction of plasmids that express E. coli beta-galactosidase in mammalian cells. Nucleic Acids Res. 17: 2365, 1989. PubMed: 2495524
Freeze dried E. coli containing the plasmid