Size (kb): 5.797
Vector: pRS423 (phagemid)
Promoters: Promoter T7 (phi10)
Construction: pJS92, pRS303, pBluescript II SK+
Construct size (kb): 5.797
Features: insert detection: lacZ'
promoter: T7 (phi10)
replicon: 2 micron
YE-type (episomal) shuttle vector
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; PvuI--3.7, 2.1.
Contains the REP3 and FRT sequences necessary for high copy propagation in yeast.
In S. cerevisiae, the copy number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 4.3 + or - 1.3% of progeny per doubling.
YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing.
The beta-galactosidase alpha peptide permits blue-white detection of recombinants.
Christianson TW, et al. Multifunctional yeast high-copy-number shuttle vectors. Gene 110: 119-122, 1992. PubMed: 1544568
component of:ATCC 87531
component of:ATCC 87535
Freeze dried phagemid in E. coli