pSV2-gpt (ATCC® 37145)

Applications: Expression vector Shuttle vector  /  Depositors: P Berg

Permits and Restrictions

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Designations pSV2-gpt
Depositors P Berg
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host Escherichia coli HB101
Vector Information
Size (kb): 5.50
Vector: pSV2-gpt (plasmid)
Promoters: Promoter SV40
Construction: SV40, pBR322, Escherichia coli
Marker(s): ampR,gpt+
Construct size (kb): 5.5

ampR, gpt+
promoter: SV40
replicon: pMB1
terminator: SV40 early
Expression vector
Shuttle vector
Restriction digests of the clone give the following sizes (kb): HindIII/BglII--5.2, 0.12; HindIII--5.2; HindIII/EcoRV--4.7, 0.58; PvuII--5.3; PstI--4.3, 0.9; PvuII/PstI--2.8, 1.5, 0.9.
Plasmid containing cells use xanthine as a guanine nucleotide precursor.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C

Mulligan RC, Berg P. Expression of a bacterial gene in mammalian cells. Science 209: 1422-1427, 1980. PubMed: 6251549

Mulligan RC, Berg P. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Proc. Natl. Acad. Sci. USA 78: 2072-2076, 1981. PubMed: 7017722

Shipped frozen