pSV2-gpt (ATCC® 37145)

Applications: Expression vector Shuttle vector  /  Depositors: P Berg

Designations pSV2-gpt
Permits and Restrictions

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Depositors P Berg
Biosafety Level 1
Host Escherichia coli HB101
Vector Information
Size (kb): 5.50
Vector: pSV2-gpt (plasmid)
Promoters: Promoter SV40
Construction: SV40, pBR322, Escherichia coli
Marker(s): ampR,gpt+
Construct size (kb): 5.5

ampR, gpt+
promoter: SV40
replicon: pMB1
terminator: SV40 early
Expression vector
Shuttle vector
Restriction digests of the clone give the following sizes (kb): HindIII/BglII--5.2, 0.12; HindIII--5.2; HindIII/EcoRV--4.7, 0.58; PvuII--5.3; PstI--4.3, 0.9; PvuII/PstI--2.8, 1.5, 0.9.
Plasmid containing cells use xanthine as a guanine nucleotide precursor.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C

Mulligan RC, Berg P. Expression of a bacterial gene in mammalian cells. Science 209: 1422-1427, 1980. PubMed: 6251549

Mulligan RC, Berg P. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Proc. Natl. Acad. Sci. USA 78: 2072-2076, 1981. PubMed: 7017722

Shipped frozen