Acanthamoeba castellanii (Douglas) Page (ATCC® 30011)

Depositor: A Castellani  /  Biosafety Level: 1

Biochemical and molecular characterization
characterization of Acanthamoeba polyphaga
Molecular characterization of corneal pathogen
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation Yeast culture, London, 1930
Product Format frozen
Storage Conditions Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:

Live Cultures:
See Protocols section for handling information
Type Strain yes
Two strains from human tissue
Reduction of uptake of Legionella by antimicrobial agents
Two genetic markers that distinguish pathogenic and nonpathogenic strains
Restriction fragment length polymorphisms of DNA
Pathogenicity and isoenzyme profiles
Biochemical and molecular characterization
Mitochondrial DNA fingerprinting
Adherence characteristics
Characterization of Acanthamoeba polyphaga
Temperature-dependent replication of Legionella pneumophila
Molecular characterization of corneal pathogen
Medium ATCC® Medium 997: Fresh water ameba medium
ATCC® Medium 711: PYB
Growth Conditions
Temperature: 25°C
Culture System: Xenic
Cryopreservation Reagents
Cryoprotective Solution
DMSO, 1.5 mL
Dryl's solution (or similar), 8.5 mL

Harvest and Preservation
  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest cells from a culture which is at or near peak density by adding 5 mL ATCC medium 5080 (Dryl's solution) and washing cells into suspension. Rub the surface of the plate with a spread bar to detach adhering trophozoites.
  3. Adjust the concentration of cells to at least 2 x 106/mL in fresh medium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place the vial in a 35°C water bath. Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial. Immediately after thawing, aseptically transfer the contents of the ampule to the center of a fresh plate of ATCC medium 997. Distribute the material evenly over the plate using a spread bar.
  9. Wrap the entire edge of the plate with parafilm and incubate upright at 25°C. Follow the protocol for maintenance of culture. 
Name of Depositor A Castellani
Year of Origin 1930

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type strain

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