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Saccharomyces cerevisiae Meyen ex E.C. Hansen (ATCC® 200897™)

Alternate State: Candida robusta Diddens et Lodder  /  Strain Designations: BY4735  /  Product Format: frozen

Deposited As Saccharomyces cerevisiae Hansen, teleomorph
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Strain Designations BY4735
Alternate State Candida robusta Diddens et Lodder
Application
transformation host with non-revertable mutations for auxotrophic markers
transformation host with non-revertable designer deletions
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen
Type Strain no
Genotype MATalpha ade2delta::hisG his3delta200 leu2delta0 met15delta0 trp1delta63 ura3delta0 RefBrachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801, (The red color phenotype of this strain is not obvious. There may be hidden mutations in the adenine biosynthesis pathway.)
Preceptrol® no
Mating Type alpha
Ploidy haploid
Comments
One of a series of strains (ATCC 200866-200902) useful for gene disruption experiments.
The strains differ in mating type and in non-revertable mutations for one or more of these auxotrophic markers: ade2delta::hisG, his3delta200, leu2delta0, lys2delta0, met15delta0, trp1delta63, ura3delta0.
Medium ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 25.0°C
Name of Depositor JD Boeke
Special Collection NCRR Contract
References

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

The red color phenotype of this strain is not obvious. There may be hidden mutations in the adenine biosynthesis pathway.

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

The red color phenotype of this strain is not obvious. There may be hidden mutations in the adenine biosynthesis pathway.