Pantoea ananatis (Serrano) Mergaert et al.

1. Open vial.
2. Rehydrate the entire pellet with approximately 0.5 mL of #3 broth. Aseptically transfer the entire contents to a 5-6 mL tube of # 3 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
3. Use several drops of the primary broth tube to inoculate a #3 plate and/or #3 agar slant.
4. Incubate at 26°C for 48 hours.

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