Additional Cell Line Authentication References

Resources to aide in your Cell Line Authentication Research

Effects of Microbial Contamination, Cross-Contamination and Misidentification

American Type Culture Collection Standards Development Organization Workgroup ASN-0002. Cell line misidentification: the beginning of the end. Nature Reviews. Cancer. 10 (6):441-448. (2010) PMID: 20448633M

Boonstra JJ et al. Verification and unmasking of widely used human esophageal adenocarcinoma cell lines. Journal of the National Cancer Institute. 102(4):271-274. (2010) PMID: 20075370

Bubenik J. Cross-contamination of cell lines in culture. Folia Biologica. 46 (5):163-164. (2000) PMID: 6451928

Buehring GC et al. Cell Line cross-contamination: how aware are mammalian cell culturists of the problems and how to monitor it? In Vitro Cellular and Developmental Biology. Animal; 40 (7):211-215. (2004) PMID: 15638703

Drexler HG et al. Mix-ups and mycoplasma: the enemies within. Leukemia Research. 26 (4):329-333. (2002) PMID: 11839374

Drexler HG et al. False leukemia-lymphoma cell lines: An update on over 500 cell lines. Leukemia. 17 (2):416-426. (2003) PMID: 12592342

Garnick RL et al. Raw materials as a source of contamination in large-scale cell culture. Developments in Biological Standardization. 93:21-29. (1998) PMID: 9737373

Kagemann G et al. Impact of Mycoplasma hyorhinis infection on L-arginine metabolism: differential regulation of the human and murine iNOS gene. Biological Chemistry. 386 (10):1055-1063. (2005) PMID: 16218877

Langdon SP et al. Cell culture contamination: an overview. Methods in Molecular Medicine. 88:309-317. (2004)

Lincoln CK et al. Cell culture contamination: sources, consequences, prevention, and elimination. Methods in Cell Biology. 57:49-65. (1998) PMID: 9648099

MacLeod RA et al. Widespread intraspecies cross-contamination of human tumor cell lines arising at source. International Journal of Cancer. 83 (4):555-563. (1999) PMID: 10508494

Masters JR. HeLa cells 50 years on: the good, the bad and the ugly. Nature Reviews. 2:315-319. (2002) PMID: 12001993

Masters JR. Human Cell Cross-contamination Since 1983. In Vitro Cellular & Dev Bio – Animal, 40:10-A. (2004)

Melcher R et al. SKY and genetic fingerprinting reveal a crosscontamination of the putative normal colon epithelial cell line NCOL-1. Cancer Genetics and Cytogenetics. 151(1):84-87. (2005) PMID: 15771911

Mirjalili A et al. Microbial contamination of cell cultures: a 2-years study. Biologicals. 33 (2):81-85. (2005) PMID: 15939285

Thompson EW et al. LCC15-MB cells are MDA-MB-435: A review of misidentified breast and prostate cell lines. Clinical & Experimental Metastasis. 21:535-541. (2004) PMID: 15679051

Wenzel U et al. Reconsidering cell line cross-contamination in NCOL-1. Cancer Genetics and Cytogenetics. 163 (1):95-96. (2005) PMID: 16271966

No Authors listed. Contamination of cell lines—a conspiracy of silence. Lancet Oncology. 2 (7):393. (2001) PMID: 11905730

Effects of Long-Term Culturing

Behrens I et al. Do cell culture conditions influence the carrier-mediated transport of peptides in Caco-2 cell monolayers? European Journal of Pharmaceutical Sciences. 19 (5):433-442. (2003) PMID: 12907294

Briske-Anderson MJ et al. Influence of culture time and passage number on morphological and physiological development of Caco-2 cells. Proceedings of the Society for Experimental Biology and Medicine. 214 (3):248-257. (1997) PMID: 9083258

Chang-Liu CM et al. Effect of passage number on cellular response to DNA-damaging agents: cell survival and gene expression. Cancer Letters. 26 (113):77-86. (1997) PMID: 9065805

Esquenet M et al. LNCaP prostatic adenocarncinoma cells derived from low and high passage numbers display divergent responses not only to androgens but also to retinoids. Journal of Steroid Biochemistry and Molecular Biology. 62:391-399. (1997) PMID: 9449242

Lin Hk et al. Supression versus induction of androgen receptor functions by the phosphatidylinositol 3-kinase/Akt pathway in prostate cancer LNCaP cells with different passage numbers. Journal of Biological Chemistry. 278 (51):50902-50907. (2003) PMID: 14555644

O’Driscoll L et al. Phenotypic and global gene expression profile changes between low passage and high passage MIN-6 cells. Journal of Endocrinology. 191 (3):665-676. (2006) PMID: 17170223

Sambuy Y et al. The Caco-2 cell line as a model of the intestinal barrier; influence of cell and culture-related factors on Caco-2 cell functional characteristics. Cell Biology and Toxicology. 21:1-26. (2005) PMID: 15868485

Vierck JL et al. Interpretation of cell culture phenomena. Methods in Cell Science. 22 (1):79-81. (2000)

Wenger SL et al. Comparison of established cell lines at different passages by karyotype and comparative genomic hybridization. Bioscience Reports. 24 (6):631-639. (2004) PMID: 16158200

Yu H et al. Evidence for diminished functional expression of intestinal transporters in Caco-2 cell monolayers at high passages. Pharmaceutical Research. 14 (6):757-762. (1997) PMID: 9210193

ICLAC Publications

A full list of references can be found in the ICLAC Register of Misidentified Cell Lines.

Masters JR, Alston-Roberts C, Barrett T, Burnett EC, Cooper JR, Dirks WG, Freshney RI, Healy L, Kerrigan L, Kohara A, Korch C, MacLeod RA, Nakamura Y, Nims RW, Reid YA, Storts DR, Capes-Davis A (2012) Cell-line authentication: End the scandal of false cell lines. Nature 492: 186. PMID: 23235867.

Capes-Davis A, Alston-Roberts C, Kerrigan L, Reid YA, Barrett T, Burnett EC, Cooper JR, Freshney RI, Healy L, Kohara A, Korch C, Masters JR, Nakamura Y, Nims RW, Storts DR, Dirks WG, Macleod RA, Drexler HG (2013) Beware imposters: MA-1, a novel MALT lymphoma cell line, is misidentified and corresponds to Pfeiffer, a diffuse large B-cell lymphoma cell line. Genes Chromosomes Cancer 52: 986-8. PMID: 23907998.

Capes-Davis A, Dirks WG, MacLeod RA, Uphoff CC (2014) Quality Matters: Cell Lines and their Use in Research. GIT Laboratory Journal Europe 17 (1-2): 12-13.

Dirks WG, Kerrigan L, Peluso C, Wilder C (2014) Choose Wisely: Obtaining Cell Lines from Reliable Sources. GIT Laboratory Journal Europe 17 (3-4): 22-24.

Cooper J, Burnett E, MacLeod RA, Nims R, Moy E, Capes-Davis A (2014) Cell Banking: Reliable Storage of Authentic Cell Lines. GIT Laboratory Journal Europe 17 (5-6): 18-20.

The International Cell Line Authentication Committee (2014) Cell Line Cross-Contamination: WSU-CLL is a known derivative of REH and is unsuitable as a model for chronic lymphocytic leukemia. Leukemia Res, Epub May 23. PMID: 24923861.

The International Cell Line Authentication Committee (2014) New Resources for an Old Problem: Cell Line Cross-Contamination. In Vitro Report 48.3.

Nardone RM, MacLeod RA, Capes-Davis A (2016) Cancer: Authenticate new xenograft models. Nature 532: 313. PMID: 27127813.

Other

Cooper et al. Species identification in cell culture: a two-pronged molecular approach. In Vitro Cell Dev Biol Anim. Nov-Dec; 43(10):344-51. Epub Oct 13. (2007) PMID: 17934781

Hartung T et al. Good cell culture practice: ECVAM good cell culture practice task force report 1, ATLA 30:407-414. (2002) PMID: 12234246

Hay RJ et al. Cell Line Preservation and Authentication in “Animal Cell Culture,” J.R.W. Masters (ed.), J. Wiley, Inc., Oxford University Press, New York City. (2000)

Hebert et. al. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society of London, Series B. (2003b) PMID: 12952648

Nardone RM. Eradication of Cross-contaminated cell lines: A call for action,. Accessed May 22, 2006. PMID: 17522957