Primary Mammary Epithelial Cells; Normal, Human (ATCC® PCS-600-010)

Organism: Homo sapiens, human  /  Tissue: Breast  / 

Organism Homo sapiens, human
Tissue Breast
Morphology Cuboidal with a characteristic pattern of swirled cells; refractile
Growth Properties Adherent
Biosafety Level 1
Disease Normal
Age Batch-specific
Gender Female
Ethnicity Batch-specific
Applications ATCC’s Human Mammary Epithelial Cells cultured in Mammary Epithelial Cell complete medium (basal medium plus growth kit) is an optimal serum-free culture model for many research areas. Common uses of HMEC include the study of breast cancer development, three dimensional culture and carcinogen screening.
Product Format frozen 1 mL
Shipping Information frozen
Storage Conditions -130°C or below
Comments A complete solution to propagate mammary epithelial cells with excellent morphology and proliferation in serum-free conditions.
Complete Growth Medium
  1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
  2. Thaw the components of the Mammary Epithelial Cell Growth Kit (PCS-600-040) just prior to adding them to the basal medium.
  3. Obtain one bottle of Mammary Epithelial Cell Basal Medium (PCS-600-030, 485 mL) from cold storage.
  4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
  5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component to the bottle of basal medium using a separate sterile pipette for each transfer.

  6. Component

    Volume

    Final Concentration

    rH-Insulin