SW 780 [SW-780, SW780] (ATCC® CRL-2169™)

Organism: Homo sapiens, human  /  Tissue: urinary bladder  / 

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Tissue
urinary bladder
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease transitional cell carcinoma
Age 80 years
Gender female
Ethnicity Caucasian
Applications
The SW 780 line was established in 1974 by A. Leibovitz from a grade I transitional cell carcinoma.
Storage Conditions liquid nitrogen vapor phase
Derivation
The SW 780 line was established in 1974 by A. Leibovitz from a grade I transitional cell carcinoma.
Clinical Data
80 years
Caucasian
female
The patient had preoperative chemotherapy (Thiotepa).
Tumorigenic Yes
Effects
Yes, forms tumors in nude mice
Comments
The patient had preoperative chemotherapy (Thiotepa).
The cells have a reported plating efficiency of 41%.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 100%
Population Doubling Time 38 hrs
Name of Depositor W McCombs
Year of Origin 1974
References

Kyriazis AA, et al. Histopathologic evaluation of response to treatment of human tumors grown in the nude mouse. Exp. Cell Biol. 51: 83-95, 1983. PubMed: 6840388

Kyriazis AA, et al. Morphological, biological, and biochemical characteristics of human bladder transitional cell carcinomas grown in tissue culture and in nude mice. Cancer Res. 44: 3997-4005, 1984. PubMed: 6744315

Kyriazis AP, et al. Response to ionizing radiation of human bladder transitional cell carcinomas grown in the nude mouse. Exp. Cell Biol. 53: 281-286, 1985. PubMed: 4043506

Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Cancer cell line mutation data
Restrictions These cells are distributed for research purposes only. The Scott and White Clinic releases the line subject to the following: 1) The cells or products derived from them must not be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. Commercial interests are the exclusive property of the Scott and White Clinic. 2) Any proposed commercial use of these cells or products produced by them must first be negotiated with the Scott and White Clinic, 2401 S. 31 Street, Temple, Texas 76508. Telephone (817) 774-2432
References

Kyriazis AA, et al. Histopathologic evaluation of response to treatment of human tumors grown in the nude mouse. Exp. Cell Biol. 51: 83-95, 1983. PubMed: 6840388

Kyriazis AA, et al. Morphological, biological, and biochemical characteristics of human bladder transitional cell carcinomas grown in tissue culture and in nude mice. Cancer Res. 44: 3997-4005, 1984. PubMed: 6744315

Kyriazis AP, et al. Response to ionizing radiation of human bladder transitional cell carcinomas grown in the nude mouse. Exp. Cell Biol. 53: 281-286, 1985. PubMed: 4043506

Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047